Identification of Open Reading Frames in Schizosaccharomyces pombe cDNAs

Identification of open reading frames in Schizosaccharomyces pombe cDNAs.

A total of 214 non-overlapping cDNA clones from Schizosaccharomyces pombe were selected and completely sequenced. The clones not previously reported were divided into the following three groups: 1) homologous to Saccharomyces cerevisiae genes (139 clones); 2) homologous to genes from other organisms but not to those from Sac. cerevisiae (4 clones); and 3) no similar sequences (40 clones). Among...

Abundant poly(A)-bearing RNAs that lack open reading frames in Schizosaccharomyces pombe.

We report here that 6.9% (68/987) of randomly selected cDNA clones from an S. pombe cDNA library lack apparently long open reading frames which we denote prl. One of them, prl1, was examined further because multiple bands were observed when it was used as a probe in northern blot analysis. These multiple bands appear to be derived from overlapping transcripts from both DNA strands, including no...

Automated identification of putative methyltransferases from genomic open reading frames.

We have analyzed existing methodologies and created novel methodologies for the automatic assignment of S-adenosylmethionine (AdoMet)-dependent methyltransferase functionality to genomic open reading frames based on predicted protein sequences. A large class of the AdoMet-dependent methyltransferases shares a common binding motif for the AdoMet cofactor in the form of a seven-strand twisted bet...

[Schizosaccharomyces pombe].

Identification of sam4 as a rad24 allele in Schizosaccharomyces pombe.

Fission yeast requires nutritional starvation to switch the mitotic cell cycle to sexual differentiation, but sam mutants, of which we had isolated nine alleles, mate without the starvation condition. These mutants are useful for understanding the mechanism underlying the way cells sense nutritional starvation and change the cell cycle. To identify the sam allele, we first sought phenotypes oth...